Project

The study of anionic lipoplexes, i.e. ternary systems formed by anionic lipids, cations and DNA, becomes the main research line in the project “Physicochemical properties of DNA-lipid complexes with biotechnology applications” (P09-FQM-4698). This research project was funded by Junta de Andalucía and was carried out in the Department of Applied Physics in the University of Granada (Spain) under the supervision of Dr. Alberto Martín-Molina (see abstract of the project).

This research line has continued through of 2 Microprojects from “Compromiso con la investigación y el desarrollo” supported by Campus de Excelencia Internacional BioTic Granada:

1. Mechanics properties of lipid interfaces: experiments and all-atomic simulactions (BS14-2015). Principal Investigator: Teresa del Castillo-Santaella.

2. Biophysics of phosphatidic acid interfaces (BS28-2015). Principal Investigator: Germán Luque-Caballero and Alberto Martín-Molina.

Posted in Sin categoría | Comments Off

Thesis

PhD Thesis: “Cation-mediated interaction between DNA and Anionic Lipid Surfaces: experimental and simulation study” of Germán Luque-Caballero associated to the project P09-FQM-4698. Granada, July 2014.

Posted in Sin categoría | Leave a comment

Last publications related to the project’s research line

[6]Atomic Force Microscopy as a tool to study the adsorption of DNA onto lipid interfaces“. Microscopy Research and Technique 80 (1), 11-17 (2017). G. Luque-Caballero1, J. Maldonado-Valderrama1, M. Quesada-Pérez2, A. Martín-Molina1

  1. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain
  2. Departamento de Física, Escuela Politécnica Superior de Linares, Universidad de Jaén, 23700, Linares, Jaén, Spain

[5] Experimental and theoretical study of the interactions underlying anionic lipoplexesEUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 44, S195-S195 (2015). G. Luque-Caballero1, J. Maldonado-Valderrama1, M. Quesada-Pérez2, A. Martín-Molina1

  1. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain
  2. Departamento de Física, Escuela Politécnica Superior de Linares, Universidad de Jaén, 23700, Linares, Jaén, Spain

[4] “Polyelectrolyte adsorption onto like-charged surfaces mediated by trivalent counterions: A Monte Carlo simulation study“. J. Chem. Phys. 140, 174701 (2014) G. Luque-Caballero1, A. Martín-Molina1, M. Quesada-Pérez2*

  1. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain
  2. Departamento de Física, Escuela Politécnica Superior de Linares, Universidad de Jaén, 23700, Linares, Jaén, Spain

[3] Using AFM to probe the complexation of DNA with anionic lipid mediated by Ca2+: the role of surface pressure“.  Soft Matter, 2014, 10, 2805–2815. G. Luque-Caballero, A. Martín-Molina, A. Y. Sánchez-Treviño, M. A. Rodriguez-Valverde, M. A. Cabrerizo-Vilchez, J. Maldonado-Valderrama*

  1. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain

[2] Adsorption of DNA onto anionic lipid surfaces“. ilustracion lipoplexAdv. Colloid  Interface Sci. (Volume 206, April 2014, Pages 172–185) A. Martín-Molina1*, G. Luque-Caballero1, J. Faraudo2, M. Quesada-Pérez3, J. Maldonado-Valderrama1

  1. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain
  2. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, E-08193 Bellaterra, Spain
  3. Departamento de Física, Escuela Politécnica Superior de Linares, Universidad de Jaén, 23700, Linares, Jaén, Spain

[1] “Competing forces in the interaction of polyelectrolytes with charged interfaces”. Curr. Opin. Colloid Interface Sci. Volume 18, Issue 6, December 2013, Pages 517–523. J. Faraudo1*, A. Martín-Molina2

  1. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, E-08193 Bellaterra, Spain
  2. Departamento de Física Aplicada, Universidad de Granada, Campus de Fuentenueva sn, 18071 Granada, Spain

Posted in Sin categoría | Leave a comment

News

DNA with a Twist

Researchers show that DNA supercoils are dynamic structures that can “hop” long distances, a phenomenon that could affect gene regulation.

Posted in Sin categoría | Leave a comment