{"id":106,"date":"2011-11-02T10:02:51","date_gmt":"2011-11-02T09:02:51","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=106"},"modified":"2023-08-07T12:51:18","modified_gmt":"2023-08-07T11:51:18","slug":"comparison-of-different-sample-treatments-for-the-analysis-of-ochratoxin-a-in-wine-by-capillary-hplc-with-laser-induced-fluorescence-detection","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=106","title":{"rendered":"Comparison of different sample treatments for the analysis of ochratoxin A in wine by capillary HPLC with laser-induced fluorescence detection"},"content":{"rendered":"<p>Ochratoxin A (OTA) is a mycotoxin naturally found in various foods, including wine. As OTA is considered as a possible human carcinogen, the maximum concentration for this compound has been established at 2\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup> in wine by the EU (Directive (CE) No 1881\/2006). Typically,  immunoaffinity columns have been used for its extraction. However, simpler, more efficient and less contaminant extraction  systems are demanding. In this work, dispersive liquid\u2013liquid  microextraction using ionic liquid as extractant solvent (IL-DLLME) and the  QuEChERS procedure, have been evaluated and compared for extraction of OTA in wine samples. Laser-induced fluorescence (LIF,  He\u2013Cd Laser excitation at 325\u00a0nm) coupled with capillary HPLC has been used for the determination of OTA, using a sodium  dodecyl sulfate micellar solution in the mobile phase to increase the             fluorescence intensity. Matrix-matched calibration curves  were established for both methods, obtaining LODs (3\u00d7 <em>S<\/em>\/<em>N<\/em>) of 5.2\u00a0ng\u00b7L<sup>\u22121<\/sup> and 85.7\u00a0ng\u00b7L<sup>\u22121<\/sup> for IL-DLLME and QuEChERS, respectively. Clean extracts were obtained  for white, rose and red wines with both methods, with recoveries between 88.7\u201394.2% for IL-DLLME and between  82.6\u201386.2% for QuEChERS. The precision was evaluated in terms of  repeatability (<em>n<\/em> =\u20099) and intermediate precision (<em>n<\/em> =\u200915), being\u2009\u2264\u20098.5% for IL-DLLME and\u2009\u2264\u20095.4% for QuEChERS.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Ochratoxin A (OTA) is a mycotoxin naturally found in various foods, including wine. As OTA is considered as a possible human carcinogen, the maximum concentration for this compound has been established at 2\u00a0\u03bcg\u00a0kg\u22121 in wine by the EU (Directive (CE) &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=106\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[73,77,22,24,31,86,63],"tags":[97],"class_list":["post-106","post","type-post","status-publish","format-standard","hentry","category-capillary-hplc","category-dllme","category-ionic-liquids","category-lif","category-mycotoxins","category-quechers","category-wine","tag-hplc"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/106","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=106"}],"version-history":[{"count":2,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/106\/revisions"}],"predecessor-version":[{"id":428,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/106\/revisions\/428"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=106"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=106"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=106"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}