{"id":124,"date":"2012-07-13T17:26:31","date_gmt":"2012-07-13T16:26:31","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=124"},"modified":"2023-08-07T12:46:10","modified_gmt":"2023-08-07T11:46:10","slug":"determination-of-5-nitroimidazoles-and-metabolites-in-environmental-samples-by-micellar-electrokinetic-chromatography","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=124","title":{"rendered":"Determination of 5-nitroimidazoles and metabolites in environmental samples by micellar electrokinetic chromatography"},"content":{"rendered":"<p>A method based on micellar electrokinetic chromatography (MEKC) with UV  detection has been developed for the determination of nine 5-nitroimidazoles (5-NDZs), including metabolites in  river water samples. Due to the relative insensitivity of UV detection in MEKC, a solid-phase extraction (SPE) method has  been proposed that preconcentrates water samples fiftyfold and cleans them up off-line. An on-line preconcentration  approach based on sweeping and the use of an extended light path  fused-silica capillary (64.5\u00a0cm\u2009\u00d7\u200950\u00a0\u03bcm i.d., 56\u00a0cm effective length) was  also found to improve the sensitivity of the method. Separation was carried out in &lt;21\u00a0min using 20\u00a0mM phosphate buffer  (pH 6.5) and 150\u00a0mM SDS as the background electrolyte (BGE). The  temperature of the capillary was kept constant at 20\u00b0C, a voltage of  25\u00a0kV was applied (normal mode), and a detected wavelength of 320\u00a0nm was utilized. Hydrodynamic injection (50\u00a0mbar for 15\u00a0s) of  the samples, which were dissolved in 20\u00a0mM phosphate (pH 6.5),             was employed. The limits of detection were lower than  1.1\u00a0\u03bcg\u00a0L<sup>\u22121<\/sup>. Recoveries of &gt;80% from spiked river water  samples were obtained for most of the analytes at three different  concentration levels with acceptable precision. This method could provide  an efficient and economical alternative to the use of chromatographic methods to monitor nitroimidazole residues, thus  supplementing the relatively few methods available for the analysis of  these compounds in environmental samples.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A method based on micellar electrokinetic chromatography (MEKC) with UV detection has been developed for the determination of nine 5-nitroimidazoles (5-NDZs), including metabolites in river water samples. Due to the relative insensitivity of UV detection in MEKC, a solid-phase extraction &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=124\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[66,25,88,52,58,62],"tags":[90],"class_list":["post-124","post","type-post","status-publish","format-standard","hentry","category-5-nitroimidazoles","category-mekc","category-spe","category-sweeping","category-uv-vis","category-water","tag-ce"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/124","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=124"}],"version-history":[{"count":2,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/124\/revisions"}],"predecessor-version":[{"id":426,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/124\/revisions\/426"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=124"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=124"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}