{"id":125,"date":"2012-08-01T20:15:19","date_gmt":"2012-08-01T19:15:19","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=125"},"modified":"2012-08-01T20:15:19","modified_gmt":"2012-08-01T19:15:19","slug":"analysis-of-cephalosporin-residues-in-environmental-waters-by-capillary-zone-electrophoresis-with-off-line-and-on-line-preconcentration","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=125","title":{"rendered":"Analysis of cephalosporin residues in environmental waters by capillary zone electrophoresis with off-line and on-line preconcentration"},"content":{"rendered":"<p>A sensitive and reliable method using capillary zone electrophoresis  with UV-diode array detection (CZE-DAD) has been developed and validated  for trace determination of cephalosporin antibiotics in spring water,  groundwater and river water matrices. Due to the lack of sensitivity of  the UV-Vis detection method, a solid-phase extraction (SPE) method has  been applied for off-line preconcentration and cleanup of water samples,  in combination with an on-line preconcentration methodology named large  volume sample stacking (LVSS) with polarity switching. The simultaneous  determination of five cephalosporins of veterinary use, namely  cephalexin (CFL), cephoperazone (CFPR), ceftiofur (CFTF), cephapirin  (CFP) and cephazolin (CFZ) has been accomplished in environmental water  samples using 70 mM acetate buffer, pH 7.0, applying a voltage of 22.5  kV at 25 \u00b0C and using cephadroxil (a cephalosporin for human use) as the  internal standard. For the SPE procedure a HLB sorbent column was used.  The combination of both off-line and on-line preconcentration  procedures provided a significant improvement in sensitivity, obtaining  detection limits of 0.1 \u03bcg L<small><sup>\u22121<\/sup><\/small> for CFL; 0.2 \u03bcg L<small><sup>\u22121<\/sup><\/small> for CFPR, CFTF and CFP, and 0.3 \u03bcg L<small><sup>\u22121<\/sup><\/small> for CFZ. The SPE-LVSS-CZE-DAD procedure is suitable for monitoring  these compounds in water samples with high sensitivity and precision and  satisfactory recoveries.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A sensitive and reliable method using capillary zone electrophoresis with UV-diode array detection (CZE-DAD) has been developed and validated for trace determination of cephalosporin antibiotics in spring water, groundwater and river water matrices. Due to the lack of sensitivity of &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=125\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,72,75,82,88,58,62],"tags":[90],"class_list":["post-125","post","type-post","status-publish","format-standard","hentry","category-antibiotics","category-cze","category-cephalosporins","category-lvss","category-spe","category-uv-vis","category-water","tag-ce"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/125","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=125"}],"version-history":[{"count":0,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/125\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=125"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=125"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=125"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}