{"id":134,"date":"2012-11-13T14:01:36","date_gmt":"2012-11-13T13:01:36","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=134"},"modified":"2012-11-13T14:01:36","modified_gmt":"2012-11-13T13:01:36","slug":"dispersive-liquid-liquid-microextraction-prior-to-field-amplified-sample-injection-for-the-sensitive-analysis-of-34-methylenedioxymetamphetamine-phencyclidine-and-lysergic-acid-diethylamide-by-capi","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=134","title":{"rendered":"Dispersive liquid-liquid microextraction prior to field-amplified sample injection for the sensitive analysis of 3,4- methylenedioxymetamphetamine, phencyclidine and lysergic acid diethylamide by capillary electrophoresis in human urine"},"content":{"rendered":"<p>A novel capillary zone electrophoresis (CZE) with ultraviolet detection  method has been developed and validated for the analysis of  3,4-methylenedioxymethamphetamine (MDMA), lysergic acid diethylamide  (LSD) and phencyclidine (PCP) in human urine. The separation of these  three analytes has been achieved in less than 8\u00a0min in a 72-cm effective  length capillary with 50-\u03bcm internal diameter. 100\u00a0mM NaH<sub>2<\/sub>PO<sub>4<\/sub>\/Na<sub>2<\/sub>HPO<sub>4<\/sub>,  pH 6.0 has been employed as running buffer, and the separation has been  carried out at temperature and voltage of 20\u00a0\u00b0C, and 25\u00a0kV,  respectively. The three drugs have been detected at 205\u00a0nm. Field  amplified sample injection (FASI) has been employed for on-line sample  preconcentration. FASI basically consists in a mismatch between the  electric conductivity of the sample and that of the running buffer and  it is achieved by electrokinetically injecting the sample diluted in a  solvent of lower conductivity than that of the carrier electrolyte.  Ultrapure water resulted to be the better sample solvent to reach the  greatest enhancement factor. Injection voltage and time have been  optimized to 5\u00a0kV and 20\u00a0s, respectively. The irreproducibility  associated to electrokinetic injection has been correcting by using  tetracaine as internal standard. Dispersive liquid\u2013liquid  microextraction (DLLME) has been employed as sample treatment using  experimental design and response surface methodology for the  optimization of critical variables. Linear responses were found for  MDMA, PCP and LSD in presence of urine matrix between 10.0 and 100\u00a0ng\/mL  approximately, and LODs of 1.00, 4.50, and 4.40\u00a0ng\/mL were calculated  for MDMA, PCP and LSD, respectively. The method has been successfully  applied to the analysis of the three drugs of interest in human urine  with satisfactory recovery percentages.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A novel capillary zone electrophoresis (CZE) with ultraviolet detection method has been developed and validated for the analysis of 3,4-methylenedioxymethamphetamine (MDMA), lysergic acid diethylamide (LSD) and phencyclidine (PCP) in human urine. The separation of these three analytes has been achieved &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=134\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6,72,77,11,13,80,58,60],"tags":[90],"class_list":["post-134","post","type-post","status-publish","format-standard","hentry","category-biological-fluids","category-cze","category-dllme","category-drugs","category-electrophoresis","category-fasi","category-uv-vis","category-urine","tag-ce"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/134","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=134"}],"version-history":[{"count":0,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/134\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=134"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=134"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}