{"id":142,"date":"2013-05-15T10:54:41","date_gmt":"2013-05-15T09:54:41","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=142"},"modified":"2013-05-15T10:54:41","modified_gmt":"2013-05-15T09:54:41","slug":"a-new-approach-in-sample-treatment-combined-with-uhplc-msms-for-the-determination-of-multiclass-mycotoxins-in-edible-nuts-and-seeds","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=142","title":{"rendered":"A new approach in sample treatment combined with UHPLC-MS\/MS for the determination of multiclass mycotoxins in edible nuts and seeds"},"content":{"rendered":"<p>A sensitive, simple and rapid method for the determination of fourteen  mycotoxins in nuts and seeds (including almonds, peanuts, sunflower  seeds, pumpkin seeds, walnuts, macadamia nuts, pistachios, hazelnuts and  pine nuts) has been developed using ultra-high performance liquid  chromatography coupled with tandem mass spectrometry (UHPLC-MS\/MS). The  sample treatment comprises a first step based on QuEChERS procedure for  the determination of fumonisin B<sub>1<\/sub>, fumonisin B<sub>2<\/sub>,  deoxynivalenol, fusarenon-X, T-2 and HT-2 toxin, citrinin,  sterigmatocystin, zearalenone and ochratoxin A. A subsequent clean-up  step based on the dispersive liquid\u2013liquid microextraction (DLLME) was  necessary for the determination of aflatoxins (B<sub>1<\/sub>, B<sub>2<\/sub>, G<sub>1<\/sub> and G<sub>2<\/sub>),  since their determination was not possible applying only the  QuEChERS-based extraction. The method was validated for peanuts as  representative matrix and was subsequently evaluated for the other eight  matrices. Quantification limits obtained for aflatoxins, the unique  mycotoxins legislated on these matrices, were lower than the maximum  levels allowed by the current legislation, while quantification limits  obtained for the other mycotoxins were lower than the limits usually  permitted by the legislation in other food matrices. Precision of the  method was always lower than 11%, and recoveries ranged between 60.7%  and 104.3%.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A sensitive, simple and rapid method for the determination of fourteen mycotoxins in nuts and seeds (including almonds, peanuts, sunflower seeds, pumpkin seeds, walnuts, macadamia nuts, pistachios, hazelnuts and pine nuts) has been developed using ultra-high performance liquid chromatography coupled &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=142\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[77,26,32,86,47,57],"tags":[101],"class_list":["post-142","post","type-post","status-publish","format-standard","hentry","category-dllme","category-mass-spectrometry","category-nuts","category-quechers","category-seeds","category-uhplc","tag-ms"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/142","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=142"}],"version-history":[{"count":0,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/142\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=142"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=142"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=142"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}