{"id":153,"date":"2013-09-09T09:50:32","date_gmt":"2013-09-09T08:50:32","guid":{"rendered":"http:\/\/wdb.ugr.es\/~fqm302\/blog\/?p=153"},"modified":"2013-09-09T09:50:32","modified_gmt":"2013-09-09T08:50:32","slug":"novel-solid-phase-extraction-method-for-the-analysis-of-5-nitroimidazoles-and-metabolites-in-milk-samples-by-capillary-electrophoresis","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=153","title":{"rendered":"Novel solid phase extraction method for the analysis of 5-nitroimidazoles and metabolites in milk samples by capillary electrophoresis"},"content":{"rendered":"<p>A new sample treatment has been developed for the extraction of  5-nitroimidazole (5-NDZ) drugs in milk samples previous to their  determination by micellar electrokinetic chromatography (MEKC). Fat  removing and protein precipitation were simultaneously carried out by  the addition of trichloroacetic acid (TCA) and subsequent  centrifugation. Clean-up and off-line concentration were achieved by a  novel solid-phase extraction (SPE) method employing mixed cation  exchange (MCX) cartridges, obtaining an off-line concentration factor of  18. Analyses were performed in less than 18\u00a0min employing 20\u00a0mM  phosphate buffer (pH 6.5) and 150\u00a0mM SDS as background electrolyte  (BGE). During the separation procedure a temperature of 20\u00a0\u00b0C and a  voltage of 25\u00a0kV (normal mode) were applied. Due to sweeping effects, an  on-line concentration was achieved for all the studied compounds and  detection limits lower than 1.8\u00a0\u03bcg\u00a0L<sup>\u22121<\/sup> were obtained. This method has been successfully applied to milk samples of different origins, including raw ewe milk.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A new sample treatment has been developed for the extraction of 5-nitroimidazole (5-NDZ) drugs in milk samples previous to their determination by micellar electrokinetic chromatography (MEKC). Fat removing and protein precipitation were simultaneously carried out by the addition of trichloroacetic &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=153\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[66,25,29,88,52,58],"tags":[90,108],"class_list":["post-153","post","type-post","status-publish","format-standard","hentry","category-5-nitroimidazoles","category-mekc","category-milk","category-spe","category-sweeping","category-uv-vis","tag-ce","tag-uv-vis-2"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/153","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=153"}],"version-history":[{"count":0,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/153\/revisions"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=153"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=153"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=153"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}