{"id":233,"date":"2017-01-23T15:25:07","date_gmt":"2017-01-23T14:25:07","guid":{"rendered":"http:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=233"},"modified":"2017-01-23T15:25:07","modified_gmt":"2017-01-23T14:25:07","slug":"development-of-magnetic-molecularly-imprinted-polymers-for-selective-extraction-determination-of-citrinin-in-rice-samples-by-liquid-chromatography-with-uv-diode-array-detection","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=233","title":{"rendered":"Development of magnetic molecularly imprinted polymers for selective extraction: determination of citrinin in rice samples by liquid chromatography with UV diode array detection."},"content":{"rendered":"<p>In this work, we report the synthesis of novel magnetic molecularly imprinted polymers (m-MIPs) and their application to the selective extraction of the mycotoxin citrinin (CIT) from food samples. The polymers were prepared by surface imprinting of Fe<sub>3<\/sub>O<sub>4<\/sub> nanoparticles, using 2-naphtholic acid (2-NA) as template molecule, <em class=\"EmphasisTypeItalic \">N<\/em>-3,5-<em class=\"EmphasisTypeItalic \">bis<\/em>(trifluoromethyl)phenyl-<em class=\"EmphasisTypeItalic \">N<\/em>&#8216;-4-vinylphenyl urea and methacrylamide as functional monomers and ethyleneglycol dimethacrylate as cross-linker. The resulting material was characterized by transmission electron microscopy (TEM), and X-ray diffraction (XRD) and Fourier transform infrared spectroscopies (FT-IR). The polymers were used to develop a solid-phase extraction method (m-MISPE) for the selective recovery of CIT from rice extracts prior to its determination by HPLC with UV diode array detection. The method involves ultrasound-assisted extraction of the mycotoxin from rice samples with (7:3, <em class=\"EmphasisTypeItalic \">v<\/em>\/<em class=\"EmphasisTypeItalic \">v<\/em>) methanol\/water, followed by sample cleanup and preconcentration with m-MIP. The extraction (washing and elution) conditions were optimized and their optimal values found to provide CIT recoveries of 94\u201398\u00a0% with relative standard deviations (RSD) less than 3.4\u00a0% (<em class=\"EmphasisTypeItalic \">n<\/em>\u2009=\u20093) for preconcentrated sample extracts (5\u00a0mL) fortified with the analyte at concentrations over the range 25\u2013100\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup>. Based on the results, the application of the m-MIPs facilitates the accurate and efficient determination of CIT in rice extracts.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In this work, we report the synthesis of novel magnetic molecularly imprinted polymers (m-MIPs) and their application to the selective extraction of the mycotoxin citrinin (CIT) from food samples. The polymers were prepared by surface imprinting of Fe3O4 nanoparticles, using &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=233\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[19,83,31,44,58],"tags":[],"class_list":["post-233","post","type-post","status-publish","format-standard","hentry","category-hplc","category-mips","category-mycotoxins","category-rice","category-uv-vis"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/233","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=233"}],"version-history":[{"count":1,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/233\/revisions"}],"predecessor-version":[{"id":234,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/233\/revisions\/234"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=233"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=233"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}