{"id":329,"date":"2018-02-23T16:08:52","date_gmt":"2018-02-23T15:08:52","guid":{"rendered":"http:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=329"},"modified":"2018-04-02T16:13:29","modified_gmt":"2018-04-02T15:13:29","slug":"simple-and-rapid-determination-of-5-nitroimidazoles-and-metabolites-in-fish-roe-samples-by-salting-out-assisted-liquid-liquid-extraction-and-uhplc-ms-ms","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=329","title":{"rendered":"Simple and rapid determination of 5-nitroimidazoles and metabolites in fish roe samples by salting-out assisted liquid-liquid extraction and UHPLC-MS\/MS."},"content":{"rendered":"<p>A novel multiresidue method is proposed for the determination of 12 5-nitroimidazoles and their metabolites in fish roe samples using UHPLC-MS\/MS. A salting-out assisted liquid-liquid extraction procedure was performed prior to sample analysis. The separation of compounds was accomplished using a C18 Zorbax Eclipse Plus column (50\u202fmm\u202f\u00d7\u202f2.1\u202fmm, 1.8\u202f\u00b5m) at 25\u202f\u00b0C and a mobile phase consisting of 0.025% (v\/v) aqueous formic acid and pure MeOH at a flow rate of 0.5\u202fmL\/min. Parameters involved in ionization and fragmentation were also optimized. The method was characterized in terms of linearity (R<sup>2<\/sup>\u202f\u2265\u202f0.9992), extraction efficiency (\u226568.9%), repeatability (RSD\u202f\u2264\u202f9.8%), reproducibility (RSD\u202f\u2264\u202f13.9%) and trueness (recoveries \u226581.4%). Decision limits (CC\u03b1) and detection capabilities (CC\u03b2) were obtained in the ranges 0.1\u20131.0 and 0.2\u20131.7\u202f\u00b5g\/kg, respectively.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A novel multiresidue method is proposed for the determination of 12 5-nitroimidazoles and their metabolites in fish roe samples using UHPLC-MS\/MS. A salting-out assisted liquid-liquid extraction procedure was performed prior to sample analysis. The separation of compounds was accomplished using &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=329\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[66,141,26,85,87,57],"tags":[101,104,107],"class_list":["post-329","post","type-post","status-publish","format-standard","hentry","category-5-nitroimidazoles","category-fish-roe","category-mass-spectrometry","category-p12-agr-1647","category-salle","category-uhplc","tag-ms","tag-salle","tag-uhplc"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/329","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=329"}],"version-history":[{"count":2,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/329\/revisions"}],"predecessor-version":[{"id":331,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/329\/revisions\/331"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=329"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=329"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=329"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}