{"id":453,"date":"2025-06-23T20:10:35","date_gmt":"2025-06-23T19:10:35","guid":{"rendered":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=453"},"modified":"2025-09-18T11:30:57","modified_gmt":"2025-09-18T10:30:57","slug":"addressing-chemical-risks-in-beekeeping-products-development-of-a-combined-ultrasound-assisted-extraction-and-quechers-based-method-for-the-simultaneous-determination-of-mycotoxins-and-pesticides-in","status":"publish","type":"post","link":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=453","title":{"rendered":"Addressing chemical risks in beekeeping products: development of a combined ultrasound-assisted extraction and QuEChERS-based method for the simultaneous determination of mycotoxins and pesticides in bee pollen and honey."},"content":{"rendered":"<p>This work presents an ultrasound assisted extraction (UAE) combined with a QuEChERS approach, followed by liquid chromatography-tandem mass spectrometry (LC-MS\/MS) analysis, for the determination of 20 mycotoxins and 10 pesticides in bee pollen and honey samples. The method was validated according to SANTE\/11312\/2021 guideline. Linearity was satisfactory (r\u00a0&gt;\u00a00.99), with LODs ranging from 0.03 to 13.3\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup> and LOQs from 0.11 to 44.4\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup>, except for five analytes in pollen. Trueness, expressed as apparent recoveries and evaluated at three concentration levels, was between 70 and 120\u00a0%, except for three compounds in honey and eight in pollen. RSDs for precisions were below 20\u00a0%. The method was applied to 28 honey and six pollen samples. Acetamiprid and azoxystrobin were detected in 29\u00a0% and 32\u00a0% of the honey samples, with concentration ranges of 1.26\u201316.2\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup> and 1.31\u20131.56\u00a0\u03bcg\u00a0kg<sup>\u22121<\/sup>, respectively. No contaminants were found in any of the pollen samples.<\/p>\n<p><a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-454\" src=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg-1024x510.jpg\" alt=\"\" width=\"640\" height=\"319\" srcset=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg-1024x510.jpg 1024w, https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg-300x149.jpg 300w, https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg-768x383.jpg 768w, https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg-1536x765.jpg 1536w, https:\/\/wpd.ugr.es\/~fqm302\/blog\/wp-content\/uploads\/2025\/06\/1-s2.0-S0956713525003731-ga1_lrg.jpg 1778w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>This work presents an ultrasound assisted extraction (UAE) combined with a QuEChERS approach, followed by liquid chromatography-tandem mass spectrometry (LC-MS\/MS) analysis, for the determination of 20 mycotoxins and 10 pesticides in bee pollen and honey samples. The method was validated &hellip; <a href=\"https:\/\/wpd.ugr.es\/~fqm302\/blog\/?p=453\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[20,26,31,171,174,165,86,57],"tags":[101,103,107],"class_list":["post-453","post","type-post","status-publish","format-standard","hentry","category-honey","category-mass-spectrometry","category-mycotoxins","category-pid2021-127804ob-i00","category-proyexcel_00195","category-pollen","category-quechers","category-uhplc","tag-ms","tag-quechers","tag-uhplc"],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=453"}],"version-history":[{"count":2,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/453\/revisions"}],"predecessor-version":[{"id":456,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=\/wp\/v2\/posts\/453\/revisions\/456"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=453"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=453"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wpd.ugr.es\/~fqm302\/blog\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}