{"id":2464,"date":"2014-10-08T13:23:52","date_gmt":"2014-10-08T11:23:52","guid":{"rendered":"http:\/\/wdb.ugr.es\/~luisca\/?page_id=2464"},"modified":"2021-03-25T10:38:59","modified_gmt":"2021-03-25T09:38:59","slug":"resources","status":"publish","type":"page","link":"https:\/\/wpd.ugr.es\/~luisca\/resources\/","title":{"rendered":"Resources"},"content":{"rendered":"<p>We have established, together with Prof. Acu\u00f1a-Castroviejo and Prof. Escames, an instrumentation platform to catalyze our research studies on mitochondrial function in health and disease. This is complemented with highly costs instruments available in the central facilities of the University of Granada.<\/p>\n<div class=\"omsc-clear\" style=\"height:30px\"><\/div>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2470\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/UHPLC-206x300.jpeg\" alt=\"UHPLC\" width=\"206\" height=\"300\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>UltiMate UHPLC with ECD and diode\u200b<\/h3>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2470\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/Electrophoresis-300x300.jpeg\" alt=\"Electrophoresis\" width=\"300\" height=\"300\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Electrophoresis and transfer systems \/ ChemiDoc Image station<\/h3>\n<ul>\n<li>Protein quantification by western-blot.\u200b<\/li>\n<li>Blue Native Gel Electrophoresis for mitochondrial supercomplexes detection.\u200b<\/li>\n<li>Complex I in gel activity.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2470\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/Lionheart-300x146.jpeg\" alt=\"Lionheart\" width=\"300\" height=\"146\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Lionheart FX Automated Microscope\u200b<\/h3>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2470\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/Nikon-300x210.jpeg\" alt=\"Nikon\" width=\"300\" height=\"210 \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Nikon Eclipse Ni Microscope\u200b<\/h3>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignright size-medium wp-image-2472\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/seahorse-300x295.jpg\" alt=\"Seahorse XF24 Analyzer\" width=\"300\" height=\"295\" srcset=\"https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/seahorse-300x295.jpg 300w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/seahorse.jpg 540w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Seahorse XF24 Analyzer<\/h3>\n<ul>\n<li>Live cell and mitochondrial bioenergetics.<\/li>\n<li>High-throughput, 24-well microplate format.<\/li>\n<li>Respiration and glycolysis measurements.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-full wp-image-2471\" style=\"margin-top: 40px; float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/oroboros.jpg\" alt=\"Oroboros instrument\" width=\"180\" height=\"157\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Oroboros instrument<\/h3>\n<ul>\n<li>High resolution mitochondrial respiration.<\/li>\n<li>Two chambers for measurements in cells, isolated mitochondria and muscle fibers.<\/li>\n<li>Respiration and membrane potential.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-full wp-image-2473\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2018\/01\/Q-Exactive.jpg\" alt=\"Q Exactive\u2122 Focus Hybrid Quadrupole-Orbitrap MS\" width=\"300\" height=\"241\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Q Exactive\u2122 Focus Hybrid Quadrupole-Orbitrap MS <\/h3>\n<ul>\n<li>Targeted Metabolomics.<\/li>\n<li>Un-targeted Metabolomics .<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-full wp-image-2473\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/shimadzu.jpg\" alt=\"Shimadzu and Gilson HPLC systems\" width=\"300\" height=\"241\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Shimadzu and Gilson HPLC systems<\/h3>\n<ul>\n<li>UV and fluorescence detectors for measuring ATP, ADP, AMP, melatonin and MDA levels.<\/li>\n<li>ESA Colouchem III electrochemical detector for measuring CoQ10 and CoQ9 levels.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2475\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/stratagene-300x296.jpeg\" alt=\"Stratagene Mx3005p RT-PCR system\" width=\"300\" height=\"296\" srcset=\"https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/stratagene-300x296.jpeg 300w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/stratagene.jpeg 394w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Stratagene Mx3005p RT-PCR system<\/h3>\n<ul>\n<li>Gene expression of mitochondrial-related genes<\/li>\n<li>Mitochondrial DNA (mtDNA) copy number<\/li>\n<li>SNP genotipyng<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div>\n<p><div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-full wp-image-2467\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/centrifuges.jpg\" alt=\"Centrifuges and ultracentrifuges\" width=\"225\" height=\"266\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Centrifuges and ultracentrifuges<\/h3>\n<ul>\n<li>Subcellular fractionation and mitocondrial isolation.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2480\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/electro-300x300.jpg\" alt=\"Electrophoresis and transfer systems \/ Kodak Image station \" width=\"300\" height=\"300\" srcset=\"https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/electro-300x300.jpg 300w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/electro-150x150.jpg 150w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/electro-144x144.jpg 144w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/electro.jpg 540w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Electrophoresis and transfer systems \/ Kodak Image station<\/h3>\n<ul>\n<li>Protein quantification by western-blot.<\/li>\n<li>Blue Native Gel Electrophoresis for mitochondrial supercomplexes detection.<\/li>\n<li>Complex I in gel activity.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2468\" style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/cuvette-300x268.jpg\" alt=\"Cuvette and plate spectrophotometers and spectrofluorometers\" width=\"300\" height=\"268\" srcset=\"https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/cuvette-300x268.jpg 300w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/cuvette.jpg 764w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Cuvette and plate spectrophotometers and spectrofluorometers<\/h3>\n<ul>\n<li>Mitochondrial complexes activities.<\/li>\n<li>Mitochondrial membrane potential.<\/li>\n<li>Antioxidant systems (Mn-SOD, glutathione).<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<p><div class=\"omsc-divider omsc-border-small omsc-style-dotted\" style=\"\"><\/div><br \/>\n<div class=\"omsc-one-half\"><img decoding=\"async\" loading=\"lazy\" class=\"lazyload alignleft size-medium wp-image-2474\"  style=\"float: right;\" data-original=\"http:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/smart-300x300.jpg\" alt=\"Smart system and treadmill for mice\" width=\"300\" height=\"300\" srcset=\"https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/smart-300x300.jpg 300w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/smart-150x150.jpg 150w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/smart-144x144.jpg 144w, https:\/\/wpd.ugr.es\/~luisca\/wp-content\/uploads\/2014\/10\/smart.jpg 540w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><div class=\"clear\"><\/div><\/div><br \/>\n<div class=\"omsc-one-half omsc-last\">\n<h3>Smart system and treadmill for mice<\/h3>\n<ul>\n<li>Evaluation of mouse locomotor activity.<\/li>\n<li>Mouse exercise training.<\/li>\n<\/ul>\n<p><div class=\"clear\"><\/div><\/div><div class=\"omsc-clear\"><\/div><\/p>\n<div style=\"clear: both;\"><\/div>\n<h3 style=\"margin-left: 300px;\">Central facilities<\/h3>\n<ul style=\"margin-left: 300px;\">\n<li>Mas spec<\/li>\n<li>Electron microscopy<\/li>\n<li>Flow citometry<\/li>\n<li>MRI for small rodents<\/li>\n<li>Animal facility with SPF zone<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>We have established, together with Prof. Acu\u00f1a-Castroviejo and Prof. Escames, an instrumentation platform to catalyze our research studies on mitochondrial function in health and disease.&hellip;<\/p>\n<p><a href=\"https:\/\/wpd.ugr.es\/~luisca\/resources\/\" class=\"more-link post-excerpt-readmore\">Read more<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/pages\/2464"}],"collection":[{"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/comments?post=2464"}],"version-history":[{"count":28,"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/pages\/2464\/revisions"}],"predecessor-version":[{"id":3011,"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/pages\/2464\/revisions\/3011"}],"wp:attachment":[{"href":"https:\/\/wpd.ugr.es\/~luisca\/wp-json\/wp\/v2\/media?parent=2464"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}