Research Areas
The labs from AD®ULT are located in the PTS Granada. The labs are equipped to conduct genetic, molecular, biochemical, physiological and pharmacological experiments in animal models of diseases, and to make analytical determinations in human samples. AD®ULT has also access to the central facilities of the University of Granada.
Facilities of the research unit
CHROMATOGRAPHY & METABOLITES DETECTION/QUANTIFICATION
- Ultimate 3000 UHPLC + Q Exactive™ Focus Hybrid Quadrupole-Orbitrap MS.
- Ultimate 3000 UHPLC + Diode array and electrochemical detectors.
- HPLC + UV and fluorescence detectors.
- HPLC + electrochemical and UV detectors.
- SpeedVac.
ANALYSIS OF TISSUES, CELLS AND BIOLOGICAL MATERIALS
- Atomic Force Microscope.
- NanoDMA analysis.
- Raman spectroscopy.
- Microscope Nikon Ni-U.
- INSTRON Mechanical Assays.
ENERGY METABOLISM
Seahorse XF24 Analyzer.
- Live cell and mitochondrial bioenergetics.
- Zebrafish and warms bionergetics.
- High-throughput, 24-well microplate format.
- Respiration and glycolysis measurements.
Two Oroboros instruments.
- High resolution mitochondrial respiration.
- Two chambers for measurements in cells, isolated mitochondria and muscle fibers.
- Respiration and membrane potential.
MOLECULAR BIOLOGY
- Stratagene Mx3005p RT-PCR system
- QX200 Droplet Digital PCR System.
- Automated Droplet Generator.
- Biostack Neo.
- Thermocyclers
- Electrophoresis and transfer systems / Kodak Image station
CELLULAR FRACTIONATION AND ORGANELLA ISOLATION
- Homogenizer Polytron, homogenizer Stuart Scientific SS2, ultrasounds homogenizer Bandelin UX-2070, ultrasounds bath
- Centrifuges Beckman AVANTI 30, Eppendorf 5810 and Eppendorf 5810R, ultracentrifuge Beckman L-90K.
MOUSE PHENOTYPING
- Square arena with video-tracking system SMART®.
- Voluntary wheels running connected to a computer for recording of activity parameters.
- Rotarod treadmill test and grip strength meter.
- Plantar test apparatus.
- Dynamic plantar aesthesiometer.
- Organ bath and wire myograph systems.
- Telemetry System for mice and rats.
- Tail-cuff plethysmography system.
CHARACTERIZATION ZEBRAFISH AND WORM MODELS
- Zebrafish facility.
- Biosorter-Pro.
Central Facilities
(cic.ugr.es)
MOUSE CLINIC
- Animal Housing with SPF and GF areas.
- MRI for rodents.
- Ivis Spectrum.
- BS-200 Chemistry Analyzer & Hematology Analyzer.
- Facility for Special diet production.
CELL CULTURE FACILITY
- Four Laminar Flow Hood
- Four CO2 incubators for cell culture
- Microscopes OLYMPUS IX53 and CKX41
- Centrifuges Sorvall ST16R and EPPENDORF 5804R with different rotors.
- Fridge, freezer and water bath.
FACS FACILITY
- FACScan Becton Dickinson.
- FACSCanto II Becton Dickinson.
- FACScalibur Becton Dickinson.
- FACSAria III Becton Dickinson.
MICROSCOPY FACILITY
- Nikon A1 Confocal microscopy.
- Automated Upright Fluorescence Microscope.
- FEI Titan Themis 200 TEM.
- TP1020 tissue processor.
- Leica EG1150 modular tissue embedding center.
- Leica RM2125 Rotary Microtome.
- Leica CM1510S cryostat.
EXPERIMENTAL RADIOLOGY FACILITY
- BECKMAN COULTER LS 6500 Scintillation System
- Gamma Counter 1470 Wizard.
- X‐ray YXLON irradiator.
MS FACILITY
- Mass Spectrometer – LCT Premier/XE
- Waters XEVO TQ-XS.
