2025
➢ Carreira de Paula J, Solano Parada J, Rosel Miñarro JF, García Olmedo P, Orantes FJ, Osuna A, De Pablos LM. On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System. Journal of Visualized Experiments. DOI: 10.3791/68874
➢ Ruiz JL, Marin A, Carreira de Paula J, Gómez-Moracho T, García Olmedo P, Andrés-León E, Solano Parada J, Orantes Bermejo F, Osuna A, De Pablos LM. Genome drafts of Lotmaria passim strains C2 and C3 isolated from honey bees in Spain. Microbiology Resource Announcements. DOI: 10.1128/mra.00642-24
2024
➢ Roggers M, E., De Pablos LM., Sunter, J.D. Gels and cells: the Leishmania biofilm as a space and place for parasite transmission. Gels and cells: the Leishmania biofilm as a space and place for parasite transmission. Trends in Parasitology. DOI: 10.1016/j.pt.2024.08.001
➢ Carreira de Paula, J., García Olmedo, P., Gómez-Moracho, T. et al. Promastigote EPS secretion and haptomonad biofilm formation as evolutionary adaptations of trypanosomatid parasites for colonizing honeybee hosts. npj Biofilms Microbiomes10, 27 (2024). https://doi.org/10.1038/s41522-024-00492-x
2023
➢ Development of a TaqMan qPCR assay for trypanosomatid multi-species detection and quantification in insects. Barranco-Gómez O, De Paula JC, Parada JS, Gómez-Moracho T, Marfil AV, Zafra M, Orantes Bermejo FJ, Osuna A, De Pablos LM. Parasit Vectors. 2023 Feb 14;16(1):69. doi: 10.1186/s13071-023-05687-3.
2022
➢ The Haptomonad Stage of Crithidia acanthocephali in Apis mellifera Hindgut. Buendía-Abad M, García-Palencia P, de Pablos LM, Martín-Hernández R, Higes M. Vet Sci. 2022 Jun 16;9(6):298. doi: 10.3390/vetsci9060298.
➢ Exploring Honeybee Abdominal Anatomy through Micro-CT and Novel Multi-Staining Approaches.De Paula JC, Doello K, Mesas C, Kapravelou G, Cornet-Gómez A, Orantes FJ, Martínez R, Linares F, Prados JC, Porres JM, Osuna A, de Pablos LM. Insects. 2022 Jun 18;13(6):556. doi: 10.3390/insects13060556.
2021
➢ First description of Lotmaria passim and Crithidia mellificae haptomonad stages in the honeybee hindgut. Buendía-Abad M, García-Palencia P, de Pablos LM, Alunda JM, Osuna A, Martín-Hernández R, Higes M.Int J Parasitol. 2021 Aug 17:S0020-7519(21)00258-7. doi: 10.1016/j.ijpara.2021.06.005.
➢ Methods for the Isolation and Study of Exovesicle DNA from Trypanosomatid Parasites. Orrego LM, Romero R, Osuna A, De Pablos LM. Methods Mol Biol. 2021;2369:301-317. doi: 10.1007/978-1-0716-1681-9_16.
➢ Retana Moreira L, Prescilla-Ledezma A, Cornet-Gomez A, Linares F, Jódar-Reyes AB, Fernandez J, Ibarrola Vannucci AK, De Pablos LM, Osuna A. Biophysical and Biochemical Comparison of Extracellular vesicles produced by Infective and Non-Infective Stages of Trypanosoma cruzi. International Journal of Molecular Sciences. 2021. May 13;22(10):5183. doi: 10.3390/ijms22105183.
➢ Ibarrola-Vannucci AK, De Pablos LM, Retana-Moreira L, Cornet-Gómez A, Cruz-Bustos T, Schijman AG, Ramírez JL, Vílchez S, Osuna A. Characterization and functional analysis of the proteins Prohibitin 1 and 2 in Trypanosoma cruzi. Plos Neglected Tropical Diseases. 2021 Apr 8;15(4):e0009322. doi: 10.1371/journal.pntd.0009322.
2020
➢ Buendía-Abad, M, Higes. M, Martín-Hernández. R, Barrios. L, Aranzazu Meana. A, Fernández-Fernández. A, Osuna. A, De Pablos. LM. Workflow of Lotmaria passim isolation: experimental infection with a low-passage strain causes higher honeybee mortality rates than the PRA-403 reference strain. International Journal for Parasitology: Parasites and Wildlife. Dec 31;14:68-74. doi: 10.1016/j.ijppaw.2020.12.003.
2019
➢De Pablos L.M; Ferreira T.R; Dowle A; Forrester S; Parry E; Newling K; Walrad PB. (7/1). 2019. The mRNA-bound proteome of Leishmania mexicana: novel genetic insight into an ancient parasite. Molecular and cellular Proteomics. doi: 10.1074/mcp.RA118.001307
MCP Cover July 2019:https://www.mcponline.org/content/18/7.cover-expansion
2018
➢Luis Miguel De Pablos Torró; Lissette Retana Moreira; Antonio Osuna. (3/1). 2018. Extracellular Vesicles in Chagas Disease: A New Passenger for an Old Disease Frontiers in Microbiology. 1-9. ISSN 1664-302X. DOI: 10.2174/138920291902180112113521
➢Luis Miguel De Pablos Torró. (1/1). 2018. Editorial: New Insights into the Genomes of Kinetoplastid Parasites.Current Genomics. Betham. 19-2, pp.1-2. DOI: 10.1007/978-1-4939-7680-5_15
➢Víctor Seco Hidalgo; Antonio Osuna; Luis Miguel De Pablos Torró. 2018. Characterizing Cell Heterogeneity Using PCR Fingerprinting of Surface Multigene Families in Protozoan Parasites. Cellular Heterogeneity. Methods and Protocols. Springer. 1745-15, pp.277-286.doi: 10.1007/978-1-4939-7680-5_15.
➢Luis Miguel De Pablos Torró (1/1). 2018. High-throughput genetic engineering in Trypanosoma cruzi Protozoan Parasitism From Omics to Prevention and Control. Caister academic Press. pp.65-76. ISBN 978-1-910190-83-8.