- Acknowledgements: DMG thanks to the Program to strengthen research groups of the University of Granada for the postdoctoral contract and the Ministry of Economy, Innovation, Science and Employment of the Junta de Andalucía. The financial support was obtained from the Junta de Andalucía (Excellence Project Ref: P12-AGR-1647).
- Authors: D. Moreno-González, A.M. García-Campaña.
- Reference: Food Chemistry 221 (2017) 1763-1769.
The use of salting-out assisted liquid–liquid extraction (SALLE) combined with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) has been evaluated for the determination of tetracyclines in infant foods based on meat and vegetables or in milk. To obtain satisfactory extraction efficiencies for the studied analytes, several parameters affecting the SALLE procedure were optimized. Analytical performances of the method were satisfactory, obtaining limits of quantification lower than 0.48 μg kg−1 in all cases. The precision, expressed as relative standard deviation (%, RSD) was below 11.3%. The extraction efficiency for fortified samples ranged from 89.2 to 96.8%, with RSDs lower than 7.3%. Matrix effect was evaluated for all samples studied, being lower than |21|% in all cases. In relation to the low solvent consumption, the proposed methodology could be considered rapid, cheap and environmentally friendly. Its applicability has been successfully tested in a wide range of infant foods.
